S1P3/EDG-3 was detected in immersion fixed paraffin embedded sections of human spleen using Mouse Human S1P3/EDG3 Monoclonal at 15ug/ml overnight at 4°C. Before incubation with the primary antibody, tissue was subjected to heat induced epitope retrieval using Retrieval Reagent Basic. Tissue was stained using the Mouse HRP-DAB Cell & Tissue Staining Kit and counterstained with hematoxylin (blue). Specific staining was localized to the marginal zone of germinal centers.
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